| January 6th, 2021 12:36 PM | |
| vikash | Sathyabama Institute of Science and Technology M.Tech - BioTechnology SBTA5201 Advanced Molecular Biology and Genetic Engineering Syllabus Sathyabama Institute of Science and Technology M.Tech - BioTechnology SBTA5201 Advanced Molecular Biology and Genetic Engineering Syllabus SATHYABAMA INSTITUTE OF SCIENCE AND TECHNOLOGY SCHOOL OF BIO AND CHEMICAL ENGINEERING SBTA5201 ADVANCED MOLECULAR BIOLOGY AND GENETIC ENGINEERING L T P Credits Total Marks 3 * 0 3 100 UNIT 1 INTRODUCTION TO GENETIC ENGINEERING AND CLONING STRATEGIES 9 Hrs. Milestones in genetic engineering. Special molecular tools used in genetic engineering. Preparation of DNA: Isolation and purification of DNA and genes, modification of cut ends and ligation of transgene. Gene transfer techniques: physical, Chemical and biological methods. Integrative DNA transfer- Agrobacterium Ti and Ri plasmids, Protoplast fusion and organelle engineering. Expression and Characterization of transgene: genomic level, transcript level and translational level. UNIT 2 ADVANCED MOLECULAR BIOLOGY TECHNIQUES- I 9 Hrs. Hybridization techniques: Southern, Northern, Western, South western and North western. PCR Techniques: Different types including real time (syber green and Taqman chemistry).DNA fingerprinting: basic genetic principle. Single locus and multilocus DNA fingerprinting: Variable Number of Tandem repeats (VNTRs) Minisatellite sequences, Short Tandom Repeats (STRs) Micro Satellite Sequences, RFLP, RAPD, ISSR, AFLP. Genome mapping: Direct mapping, indirect mapping. UNIT 3 ADVANCED MOLACULAR BIOLOGY TECHNIQUES II 9 Hrs. DNA sequencing; Maxam and Gilbert chemical degradation method, Sanger and Coulson’s enzymatic chain termination method, Next generation sequencing, Pyrosequencing, High throughput sequencingDNA foot printing, Chromosome Walking, Chromosome Jumping. Techniques for differential gene expression: DDRT and SSH. Isolation of genes and full length cDNA: 5’ and 3’ RACE basic technique and comparison with RLM RACE. Gene Silencing Techniques; Antisence RNA technology, Micro RNA- ShRNA, siRNA, technology; Construction of siRNA vectors. Site directed mutagenesis. UNIT 4 GENETICALLY MODIFIED ORGANISMS 9 Hrs. Application of genetic engineering in food (golden rice and other improved nutrictional qualities), agriculture (herbicide resistance, salt/draught tolerance, insect resistance (Bt. protein), designer flowers), aquaculture (giant salmon), medicine (xenotransplantation and gene therapy), industry and environment (plants as bioreactors, vaccines, plantibodies, bioplastics and oil eating bacteria). Engineering novel traits in plants by antisense technology and RNAi technology, Creation of Transgenic plants- Seed terminator technology. Transgenic animals- Gene therapy. Transgenic microbes: Genetic manipulation of microorganisms. Transgenic organisms and Metabolic Engineering. Site direct mutagenesis. UNIT 5 HAZARDS AND IMPACT OF GMOs 9 Hrs. Negative impacts of genetic engineering and Present controversies on genetically modified organisms (GMOs). Biosafety Considerations: Biological risks, ethical issues, economic issues, legal issues. Biosafety regulations, norms for the release of genetically engineered organisms. Max.45 Hrs. COURSE OUTCOMES On completion of the course, student will be able to CO1 - Review the methods for analyses of gene expression at different levels CO2 - Evaluate the pros and cons of the different gene mapping techniques CO3 - Describe the methods for silencing the gene expression CO4 - Summarize the application of genetic engineering techniques in the field of medicine and transgenesis CO5 - Comment on the controversies on the use of GMO in Bioremediation, medicine, agriculture etc. CO6 - Examine and evaluate the biosafety regulations and legal issues on the use of GMO TEXT / REFERENCE BOOKS 1. Old RW & Primrose S.B, Principles of Gene Manipulation, Blackwell Scientific Pub., USA, 6th Edition, 2004. 2. Jose Cibelli, Robert P Lanza, Keith H.S. Campbell, Michael D. West, Principles of Cloning, Academic Press, 2002. 3. Ernet L Winnacker, From genes to clones, Panima publishing corporation, India, 2003. 4. S.S. Purohit, Biotechnology fundamentals and applications, Agrobios (Ind.), Jothpur, 2002. 5. Brown T.A, Gene Cloning an Introduction, VNR (UK) Co.Ltd., 1988. 6. Sambrook A. and Russell D.W., Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Lab Press, NY, USA. 7. Benjamin Lewin, Genes VIII, Oxford University Press, 2004. 8. Ray D.S., Dehardt D.T., Dressler D., Single Stranded DNA pages, Cold Spring Harbor Monograph Achiever, Vol.8. END SEMESTER EXAMINATION QUESTION PAPER PATTERN Max. Marks: 100 Exam Duration: 3 Hrs. PART A: 5 Questions of 6 marks each - No choice 30 Marks PART B: 2 Questions from each unit with internal choice, each carrying 14 marks 70 Marks |
