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Sathyabama Institute of Science and Technology M.Tech - BioTechnology SBTA7006 Advanced Bioinstrumentation Syllabus

SATHYABAMA INSTITUTE OF SCIENCE AND TECHNOLOGY

SBTA7006 ADVANCED BIOINSTRUMENTATION

UNIT 1 BASIC ANALYTICAL TECHNIQUES 9 Hrs.
Solutions – types of solutions (standard, stock, saturated). Expression of concentrations – molarity, molality, normality, mass
fraction, mass percent, ppm, ppb – interconversion. Percentage solutions and ratio solutions – problems.
Microscopy – Bright field , Dark field , Phase contrast, Fluorescence, Scanning and transmission electron microscopy,
confocal microscopy- Flow cytometry.

UNIT 2 SEPARATION TECHNIQUES 9 Hrs.
Cell disruption methods and Cell fractionation – Homogenization media, High pressure homogenization, Ultrasonication –
Protein precipitation, ammonium sulphate fractionation of proteins. Centrifugation: Principle, types and applications-
Differential, isopycnic density gradient centrifugation, Rate zonal centrifugation. Filtration: Ultrafiltration, reverse osmosis,
membrane filtration, Dialysis.

UNIT 3 SPECTROSCOPY AND CHROMATOGRAPHY 9 Hrs.
Spectroscopy – Principle, block diagram, interpretation of results and applications - UV-Visible spectrophotometer - Infrared
, Fluorescence Spectrophotometry, Atomic Absorption Spectrophotometry - Raman Spectroscopy - Mass Spectrometry,
NMR and ESR. Chromatography: Principle, methods, interpretation of results and applications- TLC, Column
Chromatography - Ion exchange, Gel Filtration and Affinity Chromatography, HPLC and GC

UNIT 4 MOLECULAR TECHNIQUES 9 Hrs.
Electrophoresis of nucleic acid and proteins: Principles, methods and applications- Agarose gel electrophoresis, Pulse field
gel electrophoresis, Capillary Electrophoresis, SDS – PAGE, IEF, 2D PAGE. Blotting techniques - principle, methods and
applications - Southern, Northern, Western, Dot, slot and Zoo blot- Immunological techniques: - Ag-Ab reactions-
Immunoelectrophoresis- ELISA, RIA.

UNIT 5 TRACER TECHNIQUES AND DIAGNOSTICS 9 Hrs.
Tracer techniques- autoradiography – radiodosimetry, XRD. Immobilization of cells and enzymes - supercritical fluids
methods- Nano biotechnology in molecular diagnosis: current techniques and applications- Biosensors- principle, methods
and applications in biotechnology- microfluidics and Biochips technology.
Max. 45 Hrs.

COURSE OUTCOMES
On completion of the course, student will be able to
CO1 - To define basic nomenclature in analytical biotechnology.
CO2 - To possess the ability to demonstrate the different analytical techniques.
CO3 - To illustrate the different techniques for better understanding.
CO4 - To interpret the results obtained from various methods of analysis.
CO5 - To speculate on the analyte based on the different analysis.
CO6 - To integrate various techniques for complex problems.

TEXT / REFERENCE BOOKS
1. Thomas G.M. Schalkhammer, Analytical Biotechnology, Springer Basel AG, 2002.
2. Rajan Katoch, Analytical Techniques in Biochemistry and Molecular Biology, Springer, 2011.
3. Keith Wilson and John Walker, Practical Biochemistry - Principles and Techniques, 5th Edition, Cambridge University
Press, 2003.
4. Biophysical Chemistry: Principles and Techniques – Upadhayay and Nath – Himalaya Publishing House, 2nd Review
Edition, 2009.

END SEMESTER EXAMINATION QUESTION PAPER PATTERN
Max. Marks: 100 Exam Duration: 3 Hrs.
PART A: 5 Questions of 6 marks each - No choice 30 Marks
PART B: 2 Questions from each unit with internal choice, each carrying 14 marks 70 Marks