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Sathyabama Institute of Science and Technology B.Tech - Chemical Engineering SCHA1601 Biochemical Engineering Syllabus

SATHYABAMA INSTITUTE OF SCIENCE AND TECHNOLOGY SCHOOL OF BIO AND CHEMICAL ENGINEERING

SCHA1601 BIOCHEMICAL ENGINEERING
L T P Credits Total Marks
3 0 0 3 100

UNIT 1 INTRODUCTION 9 Hrs.
Industrial biochemical processes with typical examples, comparing chemical and biochemical processes, development and
scope of biochemical engineering as a discipline. Industrially important microbial strains; their classification; structure;
cellular genetics.

UNIT 2 ENZYME AND ENZYME KINETICS 9 Hrs.
Nomenclature and classification and properties of Enzyme, Mechanism and Kinetics of enzymatic Reactions, Evaluation of
Kinetic Parameters, Enzyme activity and factors affecting Enzyme activity, Types of inhibition. Immobilized enzyme
technology: enzyme immobilization, Immobilized enzyme kinetics: effect of external mass transfer resistance.

UNIT 3 KINETICS OF MICROBIAL GROWTH 9 Hrs.
Microbial Growth, Phases of cell growth in batch cultures, Microbial Growth Kinetics, The Stoichiometry of Microbial
Reactions, Medium Formulation and Yield Factors, Material Balance of Cell Growth, Degree of Reduction, Fed Batch
Culture, Fed-Batch Model Formulation. Kinetics of Substrate Utilisation, Product Formation and Biomass Production in Cell
Cultures, The kinetics of substrate consumption in cellular growth, Unstructured Batch Growth Models, Structured Kinetic
Models, Compartmental models, Metabolic models, Product Formation Kinetics, Sterilization, Introduction, Applications,
Heat Sterilization. Design and analysis of biological reactors.

UNIT 4 MIXING AND MASS TRANSFER 9 Hrs.
Mixing and Mass Transfer, Macro-mixing, Micro-mixing, Methods for Characterizing Mixing, Oxygen Transfer, Gas-Liquid
Mass Transfer, Oxygen Transfer Rate, Oxygen Consumption in Cell Growth, Factors Affecting Cellular Oxygen Demand,
Measurement of kla in Continuous-Stirred-Tank Bioreactor and Airlift Bioreactor, Continuous-Stirred-Tank Bioreactor, Airlift
Bioreactor, Liquid Mixing, Types of Mixing and Stirrers Types of Flows in Agitated Tanks, The Mechanism of Mixing Power
Requirement for Mixing, Un-gassed Newtonian Fluids, Un-gassed non-Newtonian Fluids, Gassed Fluids.

UNIT 5 DOWN STREAM PROCESSING 9 Hrs.
Down stream processing: Strategies to recover and purify products; separation of insoluble products, filtration and
centrifugation; cell disruption-mechanical and non-mechanical methods; separation of soluble products: liquid-liquid
extractions, membrane separation (dialysis, ultra filtration and reverse osmosis),chromatographic separation-gel permeation
chromatography, electrophoresis, final steps in purification –crystallization and drying.
Max.45 Hrs.

COURSE OUTCOMES
On completion of the course, student will be able to
CO1 - Develop skills of the students in the area of microbiology particularly to identify microbes and their structure; able
to explain biological basics and bio processing
CO2 - Explain the principles of enzyme engineering
CO3 - Calculate and analyze the kinetic parameters for microbial growth.
CO4 - Evaluate different mass transfer operations used in biochemical industries and apply mass transfer correlations to
bioreactor design. Ability to analyze mixing processes different types of mixers
CO5 - Know and select process unit operations for product recovery and purification.
CO6 - Ability to propose bioconversion technologies of microbial cell culture for the production of value-added product.

TEXT / REFERENCE BOOKS
1. Rao D.G., Introduction to Biochemical Engineering, Tata McGraw Hill, 2008.
2. Michael Shuler and Fikret Kargi, Bioprocess Engineering: Basic Concepts, 2nd Edition, Prentice Hall, Englewood Cliffs,
NJ, 2002.
3. Syed Tanveer Ahmed Inamdar, Biochemical Engineering Principle and Concepts, 2nd Edition, PHI Learning Private
Limited, 2009.
4. Wagemann, Kurt, Advances in Biochemical Engineering / Biotechnology, Springer, 2019.

END SEMESTER EXAMINATION QUESTION PAPER PATTERN
Max. Marks: 100 Exam Duration: 3 Hrs.
Part A: 10 Questions of 2 marks each - No choice 20 Marks
Part B: 2 Questions from each unit of internal choice; each carrying 16 marks 80 Marks