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msc microbiology syllabus osmania university |
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Re: msc microbiology syllabus osmania university
As you want here I am providing Osmania University Msc Microbiology Syllabus M.Sc. (Previous) I Semester Paper I MB 101 General Microbiology (4 Units x 15 Hrs = 60 Hrs teaching) Unit I History of Microbiology. Microscopy. Structure of microbial cells: Spontaneous generation and germ theory of disease, Contributions of Antony van Leeuwenhock, Louis Pasteur, Robert Koch, Edward Jenner, Winogradsky, Beijerink, Alexander Flemming, Waksman. Developments in modern biology. Principles and working of bright field microscope, fluorescent microscope, phase contrast microscope, electron microscope. Application and importance of above microscopes. Measurement of microscopic objects. Prokaryotic cell, Eukaryotic cell, Organization and function of cellular organells Cell wall of bacteria, cell membranes, flagella, pili, capsule structure, chemical structure of peptidoglycan, protoplasts, spheroplasts, microsomes and ribosomal RNAs, Nuclear material/nucleus. Unit II Methods of sterilization: Physical methods – Dry heat, moist heat, radiation methods, filtration methods, chemical methods and their application. Concept of containment facility, sterilization at industrial level Microbial cultures: Concept of pure culture, Methods of pure culture isolation, Enrichment culturing techniques, single cell isolation, and pure culture development. Preservation and Maintenance of Microbial cultures: Repeated subculturing, preservation at low temperature, sterile soil preservation, mineral oil preservation, deep freezing and liquid nitrogen preservation, freze-drying (lyophilization). Advantages and disadvantages of each method. Unit III Identification methods and classification of bacteria: - Microscopic identification characteristics, staining methods – simple staining, differential staining, structural staining and special staining methods. Ecological identification methods, Nutritional (cultural) identification characters, chemical identification characters, biochemical identification methods, immunological characteristics, pathogenic properties identification, genetic characteristics identification. Osmania University Msc Microbiology Syllabus |
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