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Sathyabama Institute of Science and Technology B.Sc - MicroBiology SBT1002 Research Methodology Syllabus SATHYABAMA INSITUTE OF SCIENCE AND TECHNOLOGY SCHOOL OF BIO AND CHEMICAL ENGINEERING SBB1002 RESEARCH METHODOLOGY L T P Credits Total Marks 3 0 0 3 100 UNIT 1 12 Hrs. Research methodology-basic concepts-types- definition of research problems- statistical methods- Correlation and Regression, Binomial, Poisson and Normal Distributions (mean and variance applications only) - Sampling Distributions: Tests based on t, F, Z and Chi square distributions- Analysis of Variance. UNIT 2 12 Hrs. Research design-Nature and Classification of research deigns,data classification,primary and secondary data,primary and secondary data- sources-advantages- Sampling- sampling concepts and methods UNIT 3 12 Hrs. Principles of biophysical methods used for analysis. X-ray diffraction, Fluoresncence, UV, ORD/CD, visible, NMR and ESR spectroscopy, Hydrodynamic methods, Atomic absorption, Plasma emission spectroscopy. UNIT 4 12 Hrs. Principles and techniques of nucleic acid hybridization and cot curves, sequencing of proteins and nucleic acids, southern, northern and south-western blotting techniques, PCR, methods for measuring nucleic acids and protein interactions. UNIT 5 12 Hrs. Principles and applications of tracer techniques in biology, radiation dosimetry, radio-active isotopes and half-life of isotopes, effect of radiation on biological systems, autoradiography, cerenkov radiation and liquid scintillation specterometry Max Hours.60 TEXT BOOK/ REFERENCES: 1. Principles of biochemistry and molecular biology – Keith Wilson & John Walker 2. Biostatistics – Khan & Khanum END SEMESTER EXAMINATION QUESTION PAPER PATTERN Max. Marks : 100 Exam Duration : 3 Hrs. PART A : 10 questions of 2 marks each - No choice 20 Marks PART B : 2 questions from each UNIT of internal choice; each carrying 16 marks 80 Marks |
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