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Old April 27th, 2021, 09:34 AM
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Default Sathyabama Institute of Science and Technology B.Sc - MicroBiology SBT1002 Research Methodology Syllabus

Sathyabama Institute of Science and Technology B.Sc - MicroBiology SBT1002 Research Methodology Syllabus

SATHYABAMA INSITUTE OF SCIENCE AND TECHNOLOGY SCHOOL OF BIO AND CHEMICAL ENGINEERING

SBB1002 RESEARCH METHODOLOGY
L T P Credits Total Marks
3 0 0 3 100

UNIT 1 12 Hrs.
Research methodology-basic concepts-types- definition of research problems- statistical methods- Correlation and
Regression, Binomial, Poisson and Normal Distributions (mean and variance applications only) - Sampling
Distributions: Tests based on t, F, Z and Chi square distributions- Analysis of Variance.

UNIT 2 12 Hrs.
Research design-Nature and Classification of research deigns,data classification,primary and secondary data,primary
and secondary data- sources-advantages- Sampling- sampling concepts and methods

UNIT 3 12 Hrs.
Principles of biophysical methods used for analysis. X-ray diffraction, Fluoresncence, UV, ORD/CD, visible, NMR and
ESR spectroscopy, Hydrodynamic methods, Atomic absorption, Plasma emission spectroscopy.

UNIT 4 12 Hrs.
Principles and techniques of nucleic acid hybridization and cot curves, sequencing of proteins and nucleic acids,
southern, northern and south-western blotting techniques, PCR, methods for measuring nucleic acids and protein
interactions.

UNIT 5 12 Hrs.
Principles and applications of tracer techniques in biology, radiation dosimetry, radio-active isotopes and half-life of
isotopes, effect of radiation on biological systems, autoradiography, cerenkov radiation and liquid scintillation
specterometry
Max Hours.60

TEXT BOOK/ REFERENCES:
1. Principles of biochemistry and molecular biology – Keith Wilson & John Walker
2. Biostatistics – Khan & Khanum

END SEMESTER EXAMINATION QUESTION PAPER PATTERN
Max. Marks : 100 Exam Duration : 3 Hrs.
PART A : 10 questions of 2 marks each - No choice 20 Marks
PART B : 2 questions from each UNIT of internal choice; each carrying 16 marks 80 Marks
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