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Sathyabama Institute of Science and Technology B.Tech - BioTechnology SBTA1603 Design and Operation of Bioreactors Syllabus

SATHYABAMA INSTITUTE OF SCIENCE AND TECHNOLOGY SCHOOL OF BIO AND CHEMICAL ENGINEERING

SBTA1603 DESIGN AND OPERATION OF BIOREACTORS
L T P Credits Total Marks
3 * 0 3 100

UNIT 1 INTRODUCTION TO BROAD SCOPE OF BIOCHEMICAL ENGINEERING 9 Hrs.
Concept of Bioreactors, Overview of SLF and SSF. Medium optimization-Plackett Burman method. Response surface
methodology, Introduction to bioreactor configuration-Basic mode of operation batch, fed-batch and continuous reactorbioreactor
lay out-Kinetics of batch and continuous culture. Criteria of scale up and scale down of bioreactor.

UNIT 2 IDEAL CONTINUOUS STIRRED TANK BIOREACTOR 9 Hrs.
Material balance-Evaluation of Monod Kinetic parameter, Alternatives to Monod equation-Blackman, Tessier, Moser,
Contois equation -Comparison of batch and CSTB-Multiple CSTB connected in series-CSTB with cell recycling.

UNIT 3 GENERAL CHARACTERISTIC OF MODELS: 9 Hrs.
Basic concept of Linear model, dynamic, lumped, continuous, white or black box model. Unstructured and structured
models. Models with growth inhibitors- Substrate inhibition, Product inhibition-Competitive and Noncompetitive product
inhibition.

UNIT 4 AGITATION AND AERATION DESIGN 9 Hrs.
Henry’s Law - mass transfer, Two film Theory, Definitions of oxygen Transfer Rate, Oxygen Uptake Rate, Specific Oxygen
Uptake Rate. Mass transfer and rheology: Rheology of broths - impact on transfer processes Oxygen transport from the
bubble to the cell.

UNIT 5 DESIGN CONSIDERATIONS 9 Hrs.
Animal and plant cell bioreactors. Determination of kLa - Correlation for kLa. Introduction to Single Use, Bioreactors (SUBs),
Max.45 Hrs.

COURSE OUTCOMES
On completion of course, student will able to
CO1 - Study of basic working principle of bioreactors and optimization of medium preparation
CO2 - Design and operation of industrial bioreactors pipeline and working process
CO3 - Evaluate the various microbial growth kinetics and comparative analysis of bioreactors
CO4 - Discuss and evaluating the microbial growth determination models
CO5 - Critique on the validity of experimental data and measurements of mass transfer rate
CO6 - Application and Operation of industrial bioreactors for various economical important products

TEXT / REFERENCE BOOKS
1. Shuler.M.L. and Kargi.F, Bioprocess Engineering Basic concepts, Pearson Education India, 1st Edition, 2003.
2. Stanbury P.F., Whitaker A. and Hall S.J., Principles of Fermentation Technology, 2nd Edition, 1997.

END SEMESTER EXAMINATION QUESTION PAPER PATTERN
Max. Marks: 100 Exam Duration: 3 Hrs.
Part A: 10 Questions of 2 marks each - No choice 20 Marks
Part B: 2 Questions from each unit of internal choice, each carrying 16 marks 80 Marks